Primary Antibody ISO 9001|ABMIUM Verified™
ABM98725

GATA-2 (phospho Ser401) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GATA-2 (phospho Ser401) rabbit pAb from ELK Biotechnology Read more ›

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GATA-2 (phospho Ser401) rabbit pAb
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GATA-2 (phospho Ser401) rabbit pAb

Product Description

GATA-2 (phospho Ser401) rabbit pAb is an ELK Biotechnology antibody for research workflows involving GATA-2.

Background

This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009],

Additional antibody information

Specificity Phospho-GATA-2 (S401) Polyclonal Antibody detects endogenous levels of GATA-2 protein only when phosphorylated at S401.
Validation evidence captions Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GATA2 (Phospho-Ser401) Antibody. The lane on the right is blocked with the phospho peptide.

Key Facts

Product type Primary Antibody Target: GATA-2
Target GATA-2
Also known as GATA2; Endothelial transcription factor GATA-2; GATA-binding protein 2
Concentration 1 mg/ml
Working concentration Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Species reactivity Human;Monkey
Observed band 50kD
Cellular localization Nucleus.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBELISA
Dilution range Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen The antiserum was produced against synthesized peptide derived from human GATA2 around the phosphorylation site of Ser401. AA range:367-416
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Monkey
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Monkey
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GATA-2
Also known as
GATA2; Endothelial transcription factor GATA-2; GATA-binding protein 2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GATA2 around the phosphorylation site of Ser401. AA range:367-416
Observed band
50kD
Cellular localization
Nucleus.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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