GATA-2 (phospho Ser401) rabbit pAb from ELK Biotechnology Read more ›
GATA-2 (phospho Ser401) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GATA-2 in WB, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009],
| Specificity | Phospho-GATA-2 (S401) Polyclonal Antibody detects endogenous levels of GATA-2 protein only when phosphorylated at S401. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GATA2 (Phospho-Ser401) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5477 |
| Target | GATA-2 |
| Research areas | Antibody Research |
| Reactivity | Human,Monkey |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GATA2 around the phosphorylation site of Ser401. AA range:367-416 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Endothelial cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Monkey | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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