Sck rabbit pAb from ELK Biotechnology Read more ›
Sck rabbit pAb is a research-use antibody from ELK Biotechnology for studying Sck in WB, IHC workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
domain:The PID domain mediates binding to the TrkA receptor.,function:Signaling adapter that couples activated growth factor receptors to signaling pathway in neurons. Involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons.,miscellaneous:PubMed:15057824 has shown that SHC2 is poorly phosphorylated by the Trk receptors, in opposite to PubMed:12006576.,PTM:Phosphorylated on tyrosines by the Trk receptors.,similarity:Contains 1 PID domain.,similarity:Contains 1 SH2 domain.,subunit:Interacts with the Trk receptors in a phosphotyrosine-dependent manner and MEGF12. Once activated, binds to GRB2.,tissue specificity:Expressed in brain. Expressed at high level in the hypothalamus and at low level in the caudate nucleus.,
| Specificity | Sck Polyclonal Antibody detects endogenous levels of Sck protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from Jurkat cells, using SHC2 Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES5437 |
| Target | Sck |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>ErbB signaling pathway, >>Ras signaling pathway, >>Chemokine signaling pathway, >>Phospholipase D signaling pathway, >>VEGF signaling pathway, >>Focal adhesion, >>Natural killer cell mediated cytotoxicity, >>Neurotrophin signaling pathway, >>Insulin signaling pathway, >>Estrogen signaling pathway, >>Prolactin signaling pathway, >>Relaxin signaling pathway, >>Growth hormone synthesis, secretion and action, >>Alcoholism, >>Bacterial invasion of epithelial cells, >>Glioma, >>Chronic myeloid leukemia, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SHC2. AA range:261-310 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in brain. Expressed at high level in the hypothalamus and at low level in the caudate nucleus. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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