Gab 1 (phospho Tyr659) rabbit pAb from ELK Biotechnology Read more ›
Gab 1 (phospho Tyr659) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Gab 1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
GRB2 associated binding protein 1(GAB1) Homo sapiens The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
| Specificity | Phospho-Gab 1 (Y659) Polyclonal Antibody detects endogenous levels of Gab 1 protein only when phosphorylated at Y659. |
|---|---|
| Validation evidence captions | Western Blot analysis of 3T3 cells using Phospho-Gab 1 (Y659) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GAB1 (Phospho-Tyr659) Antibody | Immunofluorescence analysis of HepG2 cell, using GAB1 (Phospho-Tyr659) Antibody. The lane on the right is blocked with the GAB1 (Phospho-Tyr659) peptide. | Immunohistochemistry analysis of paraffin-embedded human breast cancer, using GAB1 (Phospho-Tyr659) Antibody. The picture on the right is blocked with the GAB1 (Phospho-Tyr659) peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5422 |
| Target | Gab 1 |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>ErbB signaling pathway, >>Ras signaling pathway, >>Phospholipase D signaling pathway, >>Neurotrophin signaling pathway, >>Bacterial invasion of epithelial cells, >>Proteoglycans in cancer, >>Renal cell carcinoma, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GAB1 around the phosphorylation site of Tyr659. AA range:644-674 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Testis,Whole embryo, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
