Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98701

ZDHHC20 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ZDHHC20 rabbit pAb from ELK Biotechnology Read more ›

ZDHHC20 rabbit pAb
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ZDHHC20 rabbit pAb

Product Description

ZDHHC20 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ZDHHC20 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catalytic activity:Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.,domain:The DHHC domain is required for palmitoyltransferase activity.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the DHHC palmitoyltransferase family.,similarity:Contains 1 DHHC-type zinc finger.,

Additional antibody information

Specificity ZDHHC20 Polyclonal Antibody detects endogenous levels of ZDHHC20 protein.
Validation evidence captions Western blot analysis of lysates from Jurkat, MCF-7, and COLO cells, using ZDHHC20 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: ZDHHC20
Collaborator ELK Biotechnology
Partner model ES5418
Target ZDHHC20
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human ZDHHC20. AA range:72-121
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system PCR rescued clones,Synovial membrane,Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ZDHHC20
Also known as
ZDHHC20; Probable palmitoyltransferase ZDHHC20; Zinc finger DHHC domain-containing protein 20; DHHC-20
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ZDHHC20. AA range:72-121
Observed band
37kD
Cellular localization
Golgi apparatus membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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