ABM98701
ZDHHC20 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ZDHHC20 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
ZDHHC20 rabbit pAb is an ELK Biotechnology antibody for research workflows involving ZDHHC20.
Background
catalytic activity:Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.,domain:The DHHC domain is required for palmitoyltransferase activity.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the DHHC palmitoyltransferase family.,similarity:Contains 1 DHHC-type zinc finger.,
Additional antibody information
| Specificity | ZDHHC20 Polyclonal Antibody detects endogenous levels of ZDHHC20 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from Jurkat, MCF-7, and COLO cells, using ZDHHC20 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: ZDHHC20
| Target | ZDHHC20 |
| Also known as | ZDHHC20; Probable palmitoyltransferase ZDHHC20; Zinc finger DHHC domain-containing protein 20; DHHC-20 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 37kD |
| Cellular localization | Golgi apparatus membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ZDHHC20. AA range:72-121 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
ZDHHC20
Also known as
ZDHHC20; Probable palmitoyltransferase ZDHHC20; Zinc finger DHHC domain-containing protein 20; DHHC-20
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ZDHHC20. AA range:72-121
Observed band
37kD
Cellular localization
Golgi apparatus membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Endoplasmic reticulum-Golgi intermediate compartment membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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