Frizzled-2 rabbit pAb from ELK Biotechnology Read more ›
Frizzled-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Frizzled-2 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
frizzled class receptor 2(FZD2) Homo sapiens This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010],
| Specificity | Frizzled-2 Polyclonal Antibody detects endogenous levels of Frizzled-2 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of MCF7 cells, using FZD2 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa cells, using FZD2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HepG2 cells using FZD2 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5417 |
| Target | Frizzled-2 |
| Research areas | >>mTOR signaling pathway, >>Wnt signaling pathway, >>Hippo signaling pathway, >>Signaling pathways regulating pluripotency of stem cells, >>Melanogenesis, >>Cushing syndrome, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases, >>Human papillomavirus infection, >>Pathways in cancer, >>Proteoglycans in cancer, >>Basal cell carcinoma, >>Breast cancer, >>Hepatocellular carcinoma, >>Gastric cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FZD2. AA range:201-250 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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