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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98691

IFIT-5 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

IFIT-5 rabbit pAb from ELK Biotechnology Read more ›

IFIT-5 rabbit pAb
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IFIT-5 rabbit pAb

Product Description

IFIT-5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying IFIT-5 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

induction:By interferons.,similarity:Belongs to the IFIT family.,similarity:Contains 8 TPR repeats.,

Additional antibody information

Specificity IFIT-5 Polyclonal Antibody detects endogenous levels of IFIT-5 protein.
Validation evidence captions Western blot analysis of the lysates from HepG2 cells using IFIT5 antibody.

Key Facts

Product typePrimary Antibody› Target: IFIT-5
Collaborator ELK Biotechnology
Partner model ES5393
Target IFIT-5
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human IFIT5. AA range:281-330
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Pancreas,Spleen,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. —
Rat — —
Mouse — —
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
IFIT-5
Also known as
IFIT5; ISG58; RI58; Interferon-induced protein with tetratricopeptide repeats 5; IFIT-5; Interferon-induced 58 kDa protein; Retinoic acid- and interferon-inducible 58 kDa protein; P58
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human IFIT5. AA range:281-330
Observed band
50kD
Cellular localization
Cell projection, ruffle membrane . Colocalized with DDX58/RIG-I at cell surface ruffles. Localizes to actin-rich protrusions from the apical cell surface.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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