Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98676

FTβ rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

FTβ rabbit pAb from ELK Biotechnology Read more ›

FTβ rabbit pAb
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FTβ rabbit pAb

Product Description

FTβ rabbit pAb is a research-use antibody from ELK Biotechnology for studying FTβ in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catalytic activity:Farnesyl diphosphate + protein-cysteine = S-farnesyl protein + diphosphate.,cofactor:Binds 1 zinc ion per subunit.,function:Catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth position from the C-terminus of several proteins. The beta subunit is responsible for peptide-binding.,similarity:Belongs to the protein prenyltransferase subunit beta family.,similarity:Contains 5 PFTB repeats.,subunit:Heterodimer of an alpha and a beta subunit.,

Additional antibody information

Specificity FTβ Polyclonal Antibody detects endogenous levels of FTβ protein.
Validation evidence captions Western blot analysis of lysates from RAW264.7 abd NIH/3T3 cells, using FNTB Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: FTβ
Collaborator ELK Biotechnology
Partner model ES5343
Target FTβ
Research areas>>Terpenoid backbone biosynthesis
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human FNTB. AA range:255-304
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Neuroblastoma,Placenta,Retina,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
FTβ
Also known as
FNTB; Protein farnesyltransferase subunit beta; FTase-beta; CAAX farnesyltransferase subunit beta; Ras proteins prenyltransferase subunit beta
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human FNTB. AA range:255-304
Observed band
49kD
Cellular localization
cytosol,microtubule associated complex,protein farnesyltransferase complex,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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