Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98662

MAST3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

MAST3 rabbit pAb from ELK Biotechnology Read more ›

MAST3 rabbit pAb
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MAST3 rabbit pAb

Product Description

MAST3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MAST3 in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,caution:It is uncertain whether Met-1, Met-171 or Met-172 is the initiator.,cofactor:Magnesium.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with PTEN.,

Additional antibody information

Specificity MAST3 Polyclonal Antibody detects endogenous levels of MAST3 protein.
Validation evidence captions Western Blot analysis of RAT-HEART cells using MAST3 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from Jurkat cells, using MAST3 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: MAST3
Collaborator ELK Biotechnology
Partner model ES5295
Target MAST3
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human MAST3. AA range:1031-1080
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
MAST3
Also known as
MAST3; KIAA0561; Microtubule-associated serine/threonine-protein kinase 3
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human MAST3. AA range:1031-1080
Observed band
143kD
Cellular localization
intracellular,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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