Shank 2 rabbit pAb from ELK Biotechnology Read more ›
Shank 2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Shank 2 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
| Specificity | Shank 2 Polyclonal Antibody detects endogenous levels of Shank 2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of RAW264.7 cells using Shank 2 Polyclonal Antibody | Western blot analysis of lysates from RAW264.7, Jurkat, HepG2, and COLO cells, using SHANK2 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using SHANK2 antibody. | Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES5277 |
| Target | Shank 2 |
| Research areas | >>Glutamatergic synapse |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SHANK2. AA range:331-380 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 3 is present in epithelial colonic cells (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
