Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98645

TDAG51 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TDAG51 rabbit pAb from ELK Biotechnology Read more ›

TDAG51 rabbit pAb
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TDAG51 rabbit pAb

Product Description

TDAG51 rabbit pAb is a research-use antibody from ELK Biotechnology for studying TDAG51 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes an evolutionarily conserved proline-histidine rich nuclear protein. The encoded protein may play an important role in the anti-apoptotic effects of insulin-like growth factor-1. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity TDAG51 Polyclonal Antibody detects endogenous levels of TDAG51 protein.
Validation evidence captions Western Blot analysis of RAW264.7 cells using TDAG51 Polyclonal Antibody | Western blot analysis of lysates from RAW264.7 cells, using PHLA1 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: TDAG51
Collaborator ELK Biotechnology
Partner model ES5256
Target TDAG51
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human PHLA1. AA range:271-320
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed with highest levels in pancreas. Strongly expressed by benign melanocytic nevi, and progressively reduced expressed in primary and metastatic melanomas (at protein level).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
TDAG51
Also known as
PHLDA1; PHRIP; TDAG51; Pleckstrin homology-like domain family A member 1; Apoptosis-associated nuclear protein; Proline- and glutamine-rich protein; PQ-rich protein; PQR protein; Proline- and histidine-rich protein; T-cell death-associated
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human PHLA1. AA range:271-320
Observed band
40kD
Cellular localization
Cytoplasm . Cytoplasmic vesicle . Nucleus, nucleolus . Colocalizes with intracellular vesicles. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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