Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98638

FGF-13 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

FGF-13 rabbit pAb from ELK Biotechnology Read more ›

FGF-13 rabbit pAb
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FGF-13 rabbit pAb

Product Description

FGF-13 rabbit pAb is a research-use antibody from ELK Biotechnology for studying FGF-13 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located in a region on chromosome X, which is associated with Borjeson-Forssman-Lehmann syndrome (BFLS), making it a possible candidate gene for familial cases of the BFLS, and for other syndromal and nonspecific forms of X-linked mental retardation mapping to this region. Alternative splicing of this gene at the 5' end results in several transcript variants encoding different isoforms with different N-termini. [provided by RefSeq, Nov 2008],

Additional antibody information

Specificity FGF-13 Polyclonal Antibody detects endogenous levels of FGF-13 protein.
Validation evidence captions Western Blot analysis of JK cells using FGF-13 Polyclonal Antibody diluted at 1:500 | Western blot analysis of the lysates from Jurkat cells using FGF13 antibody.

Key Facts

Product typePrimary Antibody Target: FGF-13
Collaborator ELK Biotechnology
Partner model ES5243
Target FGF-13
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human FGF13. AA range:154-203
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Ubiquitously expressed. Predominantly expressed in the nervous system.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
FGF-13
Also known as
FGF13; FHF2; Fibroblast growth factor 13; FGF-13; Fibroblast growth factor homologous factor 2; FHF-2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human FGF13. AA range:154-203
Observed band
28kD
Cellular localization
Isoform 1: Nucleus .; Isoform 2: Cytoplasm . Nucleus .; Isoform 3: Cytoplasm . Nucleus .; Isoform 4: Cytoplasm . Nucleus .; Isoform 5: Cytoplasm . Nucleus .; Cell projection, filopodium . Cell projection, growth cone . Cell projection, dendrite . Cell membrane, sarcolemma . Cytoplasm . Not secreted. Localizes to the lateral membrane and intercalated disks of myocytes. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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