Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98632

Neuropsin rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Neuropsin rabbit pAb from ELK Biotechnology Read more ›

Neuropsin rabbit pAb
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Neuropsin rabbit pAb

Product Description

Neuropsin rabbit pAb is a research-use antibody from ELK Biotechnology for studying Neuropsin in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. This gene belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this family member may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010],

Additional antibody information

Specificity Neuropsin Polyclonal Antibody detects endogenous levels of Neuropsin protein.
Validation evidence captions Western Blot analysis of Jurkat cells using Neuropsin Polyclonal Antibody | Immunofluorescence analysis of HeLa cells, using OPN5 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Jurkat cells, using OPN5 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HUVECcells using OPN5 antibody.

Key Facts

Product typePrimary Antibody Target: Neuropsin
Collaborator ELK Biotechnology
Partner model ES5229
Target Neuropsin
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB,IHC,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human OPN5. AA range:251-300
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Detected in brain and retina and cell lines derived from neural retina.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Neuropsin
Also known as
OPN5; GPR136; PGR12; TMEM13; Opsin-5; G-protein coupled receptor 136; G-protein coupled receptor PGR12; Neuropsin; Transmembrane protein 13
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human OPN5. AA range:251-300
Observed band
43kD
Cellular localization
Cell membrane ; Multi-pass membrane protein .
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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