Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98614

FANCG (phospho Ser383) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

FANCG (phospho Ser383) rabbit pAb from ELK Biotechnology Read more ›

FANCG (phospho Ser383) rabbit pAb
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FANCG (phospho Ser383) rabbit pAb

Product Description

FANCG (phospho Ser383) rabbit pAb is a research-use antibody from ELK Biotechnology for studying FANCG in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-FANCG (S383) Polyclonal Antibody detects endogenous levels of FANCG protein only when phosphorylated at S383.
Validation evidence captions Western Blot analysis of K562 cells using Phospho-FANCG (S383) Polyclonal Antibody

Key Facts

Product typePrimary Antibody Target: FANCG
Collaborator ELK Biotechnology
Partner model ES5203
Target FANCG
Research areas>>Fanconi anemia pathway
Reactivity Human,Rat,Mouse
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phospho-peptide around the phosphorylation site of human FANCG (phospho Ser383)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Highly expressed in testis and thymus. Found in lymphoblasts.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
FANCG
Also known as
FANCG; XRCC9; Fanconi anemia group G protein; Protein FACG; DNA repair protein XRCC9
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBELISA
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human FANCG (phospho Ser383)
Observed band
69kD
Cellular localization
Nucleus . Cytoplasm . The major form is nuclear. The minor form is cytoplasmic.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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