PYK2 (phospho Tyr881) rabbit pAb from ELK Biotechnology Read more ›
PYK2 (phospho Tyr881) rabbit pAb is a research-use antibody from ELK Biotechnology for studying PYK2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity t
| Specificity | Phospho-PYK2 (Y881) Polyclonal Antibody detects endogenous levels of PYK2 protein only when phosphorylated at Y881. |
|---|---|
| Validation evidence captions | Western Blot analysis of mouse cells using Phospho-PYK2 (Y881) Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PYK2 (Phospho-Tyr881) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr881) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5196 |
| Target | PYK2 |
| Research areas | >>Calcium signaling pathway, >>Chemokine signaling pathway, >>Phospholipase D signaling pathway, >>Natural killer cell mediated cytotoxicity, >>Leukocyte transendothelial migration, >>GnRH signaling pathway, >>Yersinia infection, >>Hepatitis B, >>Human cytomegalovirus infection, >>Human immunodeficiency virus 1 infection |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PYK2 around the phosphorylation site of Tyr881. AA range:847-896 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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