Cleaved-Thrombin R (S42) rabbit pAb from ELK Biotechnology Read more ›
Cleaved-Thrombin R (S42) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Thrombin R in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015],
| Specificity | Cleaved-Thrombin R (S42) Polyclonal Antibody detects endogenous levels of fragment of activated Thrombin R protein resulting from cleavage adjacent to S42. |
|---|---|
| Validation evidence captions | Western Blot analysis of Jurkat cells using Cleaved-Thrombin R (S42) Polyclonal Antibody | Western blot analysis of lysates from Jurkat cells, using PAR1 (Cleaved-Ser42) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5185 |
| Target | Thrombin R |
| Research areas | >>Rap1 signaling pathway, >>Calcium signaling pathway, >>cAMP signaling pathway, >>Phospholipase D signaling pathway, >>Neuroactive ligand-receptor interaction, >>PI3K-Akt signaling pathway, >>Complement and coagulation cascades, >>Platelet activation, >>Regulation of actin cytoskeleton, >>Pathogenic Escherichia coli infection, >>Pathways in cancer |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PAR1. AA range:23-72 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Platelets and vascular endothelial cells. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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