ERβ (phospho Ser105) rabbit pAb
ERβ (phospho Ser105) rabbit pAb from ELK Biotechnology Read more ›
Product Description
ERβ (phospho Ser105) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Estrogen Receptor-β.
Background
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-ERβ (S105) Polyclonal Antibody detects endogenous levels of ERβ protein only when phosphorylated at S105. |
|---|---|
| Validation evidence captions | Western Blot analysis of HepG2 cells using Phospho-ERβ (S105) Polyclonal Antibody | Immunofluorescence analysis of HUVEC cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells, using Estrogen Receptor-beta (Phospho-Ser105) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | Estrogen Receptor-β |
| Also known as | ESR2; ESTRB; NR3A2; Estrogen receptor beta; ER-beta; Nuclear receptor subfamily 3 group A member 2 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 59kD |
| Cellular localization | Nucleus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Endocrine resistance;>>Estrogen signaling pathway;>>Prolactin signaling pathway;>>GnRH secretion;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>Breast cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-beta around the phosphorylation site of Ser105. AA range:71-120 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.