Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98586

EphA7 (phospho Tyr791) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

EphA7 (phospho Tyr791) rabbit pAb from ELK Biotechnology Read more ›

EphA7 (phospho Tyr791) rabbit pAb
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EphA7 (phospho Tyr791) rabbit pAb

Product Description

EphA7 (phospho Tyr791) rabbit pAb is a research-use antibody from ELK Biotechnology for studying EPHA7 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Increased expression of this gene is associated with multiple forms of carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],

Additional antibody information

Specificity Phospho-EphA7 (Y791) Polyclonal Antibody detects endogenous levels of EphA7 protein only when phosphorylated at Y791.
Validation evidence captions Western Blot analysis of JK cells using Phospho-EphA7 (Y791) Polyclonal Antibody

Key Facts

Product typePrimary Antibody Target: EPHA7
Collaborator ELK Biotechnology
Partner model ES5129
Target EPHA7
Research areas>>Axon guidance
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phospho-peptide around the phosphorylation site of human EphA7 (phospho Tyr791)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
EPHA7
Also known as
EPHA7; EHK3; HEK11; Ephrin type-A receptor 7; EPH homology kinase 3; EHK-3; EPH-like kinase 11; EK11; hEK11
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human EphA7 (phospho Tyr791)
Observed band
112kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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