Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98570

IGSF4C rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

IGSF4C rabbit pAb from ELK Biotechnology Read more ›

IGSF4C rabbit pAb
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IGSF4C rabbit pAb

Product Description

IGSF4C rabbit pAb is a research-use antibody from ELK Biotechnology for studying IGSF4C in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.,PTM:N-glycosylated.,similarity:Belongs to the nectin family.,similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Monomer and homodimer.,tissue specificity:Expressed in brain, prostate, brain, kidney and some other organs.,

Additional antibody information

Specificity IGSF4C Polyclonal Antibody detects endogenous levels of IGSF4C protein.
Validation evidence captions Western Blot analysis of RAW264.7 cells using IGSF4C Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using CADM4 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CADM4 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from RAW264.7 cells, using CADM4 Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: IGSF4C
Collaborator ELK Biotechnology
Partner model ES5089
Target IGSF4C
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CADM4. AA range:339-388
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in brain, prostate, brain, kidney and some other organs.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
IGSF4C
Also known as
CADM4; IGSF4C; NECL4; TSLL2; Cell adhesion molecule 4; Immunoglobulin superfamily member 4C; IgSF4C; Nectin-like protein 4; NECL-4; TSLC1-like protein 2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CADM4. AA range:339-388
Observed band
48kD
Cellular localization
Membrane ; Single-pass type I membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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