HuB/D rabbit pAb from ELK Biotechnology Read more ›
HuB/D rabbit pAb is a research-use antibody from ELK Biotechnology for studying HuB/D in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
ELAV like RNA binding protein 2(ELAVL2) Homo sapiens The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs, including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010],
| Specificity | HuB/D Polyclonal Antibody detects endogenous levels of HuB/D protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of Hela cells using HuB/D Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of HepG2 cells, using ELAV2/4 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from Jurkat and HUVEC cells, using ELAV2/4 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from 293 cells using ELAV2/4 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5087 |
| Target | HuB/D |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ELAV2/4. AA range:61-110 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain; neural-specific. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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