EGFR (phospho Tyr1016) rabbit pAb from ELK Biotechnology Read more ›
EGFR (phospho Tyr1016) rabbit pAb is a research-use antibody from ELK Biotechnology for studying EGFR in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016],
| Specificity | Phospho-EGFR (Y1016) Polyclonal Antibody detects endogenous levels of EGFR protein only when phosphorylated at Y1016. |
|---|---|
| Validation evidence captions | Western Blot analysis of HELA cells using Phospho-EGFR (Y1016) Polyclonal Antibody diluted at 1:1000 | Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunofluorescence analysis of HeLa cells, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5060 |
| Target | EGFR |
| Research areas | >>EGFR tyrosine kinase inhibitor resistance, >>Endocrine resistance, >>MAPK signaling pathway, >>ErbB signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>Calcium signaling pathway, >>HIF-1 signaling pathway, >>FoxO signaling pathway, >>Phospholipase D signaling pathway, >>Endocytosis, >>PI3K-Akt signaling pathway, >>Focal adhesion, >>Adherens junction, >>Gap junction, >>JAK-STAT signaling pathway, >>Regulation of actin cytoskeleton, >>GnRH signaling pathway, >>Estrogen signaling pathway, >>Oxytocin signaling pathway, >>Relaxin signaling pathway, >>Parathyroid hormone synthesis, secretion and action, >>Cushing syndrome, >>Epithelial cell signaling in Helicobacter pylori infection, >>Shigellosis, >>Hepatitis C, >>Human cytomegalovirus infection, >>Human papillomavirus infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Proteoglycans in cancer, >>MicroRNAs in cancer, >>Chemical carcinogenesis - receptor activation, >>Chemical carcinogenesis - reactive oxygen species, >>Colorectal cance |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1016. AA range:986-1035 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
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| Human | |||||||
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| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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