Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98554

CELSR3 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CELSR3 rabbit pAb from ELK Biotechnology Read more ›

CELSR3 rabbit pAb
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CELSR3 rabbit pAb

Product Description

CELSR3 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CELSR3 in IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene belongs to the flamingo subfamily, which is included in the cadherin superfamily. The flamingo cadherins consist of nonclassic-type cadherins that do not interact with catenins. They are plasma membrane proteins containing seven epidermal growth factor-like repeats, nine cadherin domains and two laminin A G-type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic feature of their subfamily. The encoded protein may be involved in the regulation of contact-dependent neurite growth and may play a role in tumor formation. [provided by RefSeq, Jun 2013],

Additional antibody information

Specificity CELSR3 Polyclonal Antibody detects endogenous levels of CELSR3 protein.
Validation evidence captions Immunofluorescence analysis of A549 cells, using CELSR3 Antibody. The picture on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: CELSR3
Collaborator ELK Biotechnology
Partner model ES5051
Target CELSR3
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications IF,ELISA
ConjugationUnconjugated
Dilution rangeImmunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CELSR3. AA range:3195-3244
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain,Cord blood,Duodenum,Epithelium,Kidney,Uterus,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ IF
Species Dilution Notes
Human Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CELSR3
Also known as
CELSR3; CDHF11; EGFL1; FMI1; KIAA0812; MEGF2; Cadherin EGF LAG seven-pass G-type receptor 3; Cadherin family member 11; Epidermal growth factor-like protein 1; EGF-like protein 1; Flamingo homolog 1; hFmi1; Multiple epidermal growth factor-
Concentration
1 mg/ml
Working concentration
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CELSR3. AA range:3195-3244
Cellular localization
Cell membrane; Multi-pass membrane protein.
Dilution range
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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