ABM98535
DOCK 180 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
DOCK 180 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
DOCK 180 rabbit pAb is an ELK Biotechnology antibody for research workflows involving DOCK 180.
Background
This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014],
Additional antibody information
| Specificity | DOCK 180 Polyclonal Antibody detects endogenous levels of DOCK 180 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from HeLa cells, using DOCK1 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: DOCK 180
| Target | DOCK 180 |
| Also known as | DOCK1; Dedicator of cytokinesis protein 1; 180 kDa protein downstream of CRK; DOCK180 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse |
| Observed band | 215kD |
| Cellular localization | Cytoplasm . Membrane . Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Focal adhesion;>>Fc gamma R-mediated phagocytosis;>>Regulation of actin cytoskeleton;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DOCK1. AA range:1661-1710 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
DOCK 180
Also known as
DOCK1; Dedicator of cytokinesis protein 1; 180 kDa protein downstream of CRK; DOCK180
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human DOCK1. AA range:1661-1710
Observed band
215kD
Cellular localization
Cytoplasm . Membrane . Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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