Dynamin I (phospho Ser778) rabbit pAb
Dynamin I (phospho Ser778) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Dynamin I (phospho Ser778) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Dynamin I.
Background
dynamin 1(DNM1) Homo sapiens This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | Phospho-Dynamin I (S778) Polyclonal Antibody detects endogenous levels of Dynamin I protein only when phosphorylated at S778. |
|---|---|
| Validation evidence captions | Western Blot analysis of Mouse-Brain cells using Phospho-Dynamin I (S778) Polyclonal Antibody | Western blot analysis of DYN1 (Phospho-Ser778) Antibody. The lane on the right is blocked with the DYN1 (Phospho-Ser778) peptide. |
Key Facts
| Target | Dynamin I |
| Also known as | DNM1; DNM; Dynamin-1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 100kD |
| Cellular localization | Cytoplasm . Cytoplasm, cytoskeleton . Microtubule-associated. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Phospholipase D signaling pathway;>>Endocytosis;>>Synaptic vesicle cycle;>>Endocrine and other factor-regulated calcium reabsorption;>>Bacterial invasion of epithelial cells |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DYN1 around the phosphorylation site of Ser778. AA range:751-800 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.