Photomedin-1 rabbit pAb from ELK Biotechnology Read more ›
Photomedin-1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Photomedin-1 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
PTM:May be cleaved at Lys-295 after secretion.,PTM:O-glycosylated but not N-glycosylated.,similarity:Contains 1 olfactomedin-like domain.,subunit:Homodimer. Binds to heparin and chondroitin sulfate E.,
| Specificity | Photomedin-1 Polyclonal Antibody detects endogenous levels of Photomedin-1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of RAW264.7 cells using Photomedin-1 Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using OLFML2A Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from RAW264.7 cells, using OLFML2A Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES4996 |
| Target | Photomedin-1 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human OLFML2A. AA range:311-360 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | In the kidney expressed only by podocytes, wherein they localize to major processes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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