ABM98528
Photomedin-1 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Photomedin-1 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Photomedin-1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Photomedin-1.
Background
PTM:May be cleaved at Lys-295 after secretion.,PTM:O-glycosylated but not N-glycosylated.,similarity:Contains 1 olfactomedin-like domain.,subunit:Homodimer. Binds to heparin and chondroitin sulfate E.,
Additional antibody information
| Specificity | Photomedin-1 Polyclonal Antibody detects endogenous levels of Photomedin-1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of RAW264.7 cells using Photomedin-1 Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using OLFML2A Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from RAW264.7 cells, using OLFML2A Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: Photomedin-1
| Target | Photomedin-1 |
| Also known as | OLFML2A; Olfactomedin-like protein 2A; Photomedin-1 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 73kD |
| Cellular localization | Secreted . Localizes to the podocyte major processes. Colocalized with the major process protein VIM throughout podocyte development. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human OLFML2A. AA range:311-360 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Photomedin-1
Also known as
OLFML2A; Olfactomedin-like protein 2A; Photomedin-1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human OLFML2A. AA range:311-360
Observed band
73kD
Cellular localization
Secreted . Localizes to the podocyte major processes. Colocalized with the major process protein VIM throughout podocyte development. .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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