DGK-β rabbit pAb from ELK Biotechnology Read more ›
DGK-β rabbit pAb is a research-use antibody from ELK Biotechnology for studying DGK-β in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008],
| Specificity | DGK-β Polyclonal Antibody detects endogenous levels of DGK-β protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HuvEC cells using DGK-β Polyclonal Antibody | Western blot analysis of the lysates from HUVECcells using DGKB antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4958 |
| Target | DGK-β |
| Research areas | >>Glycerolipid metabolism, >>Glycerophospholipid metabolism, >>Metabolic pathways, >>Phosphatidylinositol signaling system, >>Phospholipase D signaling pathway, >>Choline metabolism in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DGKB. AA range:657-706 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 1: Specifically expressed in brain but also detected in uterus (PubMed:11719522). In adult brain, expressed in the amygdala, caudate nucleus, and hippocampus (PubMed:11719522). ; Isoform 2: More ubiquitously expressed but at lower level compared to isoform 1. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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