CYP11A1 rabbit pAb
CYP11A1 rabbit pAb from ELK Biotechnology Read more ›
Product Description
CYP11A1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CYP11A1.
Background
cytochrome P450 family 11 subfamily A member 1(CYP11A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008],
Additional antibody information
| Specificity | CYP11A1 Polyclonal Antibody detects endogenous levels of CYP11A1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HeLa cells using CYP11A1 Polyclonal Antibody | Western blot analysis of the lysates from HeLa cells using Cytochrome P450 11A1 antibody. |
Key Facts
| Target | CYP11A1 |
| Also known as | CYP11A1; CYP11A; Cholesterol side-chain cleavage enzyme; mitochondrial; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc) |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human |
| Observed band | 60kD |
| Cellular localization | Mitochondrion inner membrane ; Peripheral membrane protein . Localizes to the matrix side of the mitochondrion inner membrane. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Steroid hormone biosynthesis;>>Metabolic pathways;>>Ovarian steroidogenesis;>>Aldosterone synthesis and secretion;>>Cortisol synthesis and secretion;>>Cushing syndrome |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cytochrome P450 11A1. AA range:412-461 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.