CYP11A1 rabbit pAb from ELK Biotechnology Read more ›
CYP11A1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CYP11A1 in WB, ELISA workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
cytochrome P450 family 11 subfamily A member 1(CYP11A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008],
| Specificity | CYP11A1 Polyclonal Antibody detects endogenous levels of CYP11A1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HeLa cells using CYP11A1 Polyclonal Antibody | Western blot analysis of the lysates from HeLa cells using Cytochrome P450 11A1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4947 |
| Target | CYP11A1 |
| Research areas | >>Steroid hormone biosynthesis, >>Metabolic pathways, >>Ovarian steroidogenesis, >>Aldosterone synthesis and secretion, >>Cortisol synthesis and secretion, >>Cushing syndrome |
| Reactivity | Human |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cytochrome P450 11A1. AA range:412-461 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Choriocarcinoma,Placenta, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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