GRK 2 (phospho Ser685) rabbit pAb from ELK Biotechnology Read more ›
GRK 2 (phospho Ser685) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GRK 2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-GRK 2 (S685) Polyclonal Antibody detects endogenous levels of GRK 2 protein only when phosphorylated at S685. |
|---|---|
| Validation evidence captions | Western Blot analysis of JK cells using Phospho-GRK 2 (S685) Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from HT29 cells treated with insulin 0.01U/ml 15', using GRK2 (Phospho-Ser685) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4932 |
| Target | GRK 2 |
| Research areas | >>Chemokine signaling pathway, >>Endocytosis, >>Hedgehog signaling pathway, >>Glutamatergic synapse, >>Olfactory transduction, >>Morphine addiction |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GRK2 around the phosphorylation site of Ser685. AA range:640-689 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in peripheral blood leukocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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