CYP2C8 rabbit pAb from ELK Biotechnology Read more ›
CYP2C8 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CYP2C8 in IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010],
| Specificity | CYP2C8 Polyclonal Antibody detects endogenous levels of CYP2C8 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of Cytochrome P450 2C8 Antibody. The lane on the right is blocked with the Cytochrome P450 2C8 peptide. | Immunohistochemistry analysis of paraffin-embedded human testis, using Cytochrome P450 2C8 Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4930 |
| Target | CYP2C8 |
| Research areas | >>Arachidonic acid metabolism, >>Linoleic acid metabolism, >>Retinol metabolism, >>Drug metabolism - cytochrome P450, >>Metabolic pathways, >>Serotonergic synapse, >>Chemical carcinogenesis - DNA adducts, >>Lipid and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | IHC-p: 1/100 - 1/300. IF:1:50-200 ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CYP2C8. AA range:241-290 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Blood,Kidney,Liver, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p: 1/100 - 1/300. IF:1:50-200 ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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