CYP2C8 rabbit pAb
CYP2C8 rabbit pAb from ELK Biotechnology Read more ›
Product Description
CYP2C8 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CYP2C8.
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics, including the anticonvulsive drug mephenytoin, benzo(a)pyrene, 7-ethyoxycoumarin, and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Nov 2010],
Additional antibody information
| Specificity | CYP2C8 Polyclonal Antibody detects endogenous levels of CYP2C8 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of Cytochrome P450 2C8 Antibody. The lane on the right is blocked with the Cytochrome P450 2C8 peptide. | Immunohistochemistry analysis of paraffin-embedded human testis, using Cytochrome P450 2C8 Antibody. The picture on the right is blocked with the synthesized peptide. |
Key Facts
| Target | CYP2C8 |
| Also known as | CYP2C8; Cytochrome P450 2C8; CYPIIC8; Cytochrome P450 IIC2; Cytochrome P450 MP-12; Cytochrome P450 MP-20; Cytochrome P450 form 1; S-mephenytoin 4-hydroxylase |
| Concentration | 1 mg/ml |
| Working concentration | IHC-p: 1/100 - 1/300. IF:1:50-200 ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Cellular localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Arachidonic acid metabolism;>>Linoleic acid metabolism;>>Retinol metabolism;>>Drug metabolism - cytochrome P450;>>Metabolic pathways;>>Serotonergic synapse;>>Chemical carcinogenesis - DNA adducts;>>Lipid and atherosclerosis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
IHCIFELISA |
| Dilution range | IHC-p: 1/100 - 1/300. IF:1:50-200 ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CYP2C8. AA range:241-290 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | IHC-p: 1/100 - 1/300. IF:1:50-200 ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.