CYP3A7 rabbit pAb from ELK Biotechnology Read more ›
CYP3A7 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CYP3A7 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene and is represented by GeneID:100861540. [provided by RefSeq, Jan 2015],
| Specificity | CYP3A7 Polyclonal Antibody detects endogenous levels of CYP3A7 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293T cells using CYP3A7 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from LOVO cells, using Cytochrome P450 3A7 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from K562 cells using Cytochrome P450 3A7 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4928 |
| Target | CYP3A7 |
| Research areas | >>Steroid hormone biosynthesis, >>Retinol metabolism, >>Metabolic pathways, >>Chemical carcinogenesis - DNA adducts |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cytochrome P450 3A7. AA range:231-280 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in fetal liver (at protein level). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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