ABM98496
CYP3A7 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
CYP3A7 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
CYP3A7 rabbit pAb is an ELK Biotechnology antibody for research workflows involving CYP3A7.
Background
This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1. Naturally-occurring readthrough transcription occurs between this gene and the downstream CYP3A51P pseudogene and is represented by GeneID:100861540. [provided by RefSeq, Jan 2015],
Additional antibody information
| Specificity | CYP3A7 Polyclonal Antibody detects endogenous levels of CYP3A7 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of 293T cells using CYP3A7 Polyclonal Antibody diluted at 1:500 | Western blot analysis of lysates from LOVO cells, using Cytochrome P450 3A7 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from K562 cells using Cytochrome P450 3A7 antibody. |
Key Facts
Product type
Primary Antibody›
Target: CYP3A7
| Target | CYP3A7 |
| Also known as | CYP3A7; Cytochrome P450 3A7; CYPIIIA7; Cytochrome P450-HFLA |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 50kD |
| Cellular localization | Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane ; Peripheral membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Steroid hormone biosynthesis;>>Retinol metabolism;>>Metabolic pathways;>>Chemical carcinogenesis - DNA adducts |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cytochrome P450 3A7. AA range:231-280 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
CYP3A7
Also known as
CYP3A7; Cytochrome P450 3A7; CYPIIIA7; Cytochrome P450-HFLA
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 3A7. AA range:231-280
Observed band
50kD
Cellular localization
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane ; Peripheral membrane protein.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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