ABM98495
AR-β3 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
AR-β3 rabbit pAb from ELK Biotechnology Read more ›
Related formats
Zoom
Product Description
AR-β3 rabbit pAb is an ELK Biotechnology antibody for research workflows involving AR-β3.
Background
The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. [provided by RefSeq, Feb 2009],
Additional antibody information
| Specificity | AR-β3 Polyclonal Antibody detects endogenous levels of AR-β3 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western Blot analysis of K562 cells using AR-β3 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of 22RV1 cells using AR-β3 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from K562 cells, using ADRB3 Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
Product type
Primary Antibody›
Target: AR-β3
| Target | AR-β3 |
| Also known as | ADRB3; ADRB3R; B3AR; Beta-3 adrenergic receptor; Beta-3 adrenoreceptor; Beta-3 adrenoceptor |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 36kD |
| Cellular localization | Cell membrane; Multi-pass membrane protein. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>Neuroactive ligand-receptor interaction;>>Thermogenesis;>>Regulation of lipolysis in adipocytes;>>Renin secretion;>>Salivary secretion;>>Chemical carcinogenesis - receptor activation |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIFELISA |
| Dilution range | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADRB3. AA range:250-299 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Why researchers trust ABMIUM
2,000+
Researchers trust us
50,000+
Citations
ISO 9001
Certified Collaborators
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
AR-β3
Also known as
ADRB3; ADRB3R; B3AR; Beta-3 adrenergic receptor; Beta-3 adrenoreceptor; Beta-3 adrenoceptor
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human ADRB3. AA range:250-299
Observed band
36kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Dilution range
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
Customer Reviews
Recently Viewed