AR-β2 (phospho Ser346) rabbit pAb from ELK Biotechnology Read more ›
AR-β2 (phospho Ser346) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Adrenergic Receptor β2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-AR-β2 (S346) Polyclonal Antibody detects endogenous levels of AR-β2 protein only when phosphorylated at S346. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody | Immunofluorescence analysis of HeLa cells, using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HepG2 cells treated with nocodazole 1ug/ml 16h, using Adrenergic Receptor beta2 (Phospho-Ser346) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4919 |
| Target | Adrenergic Receptor β2 |
| Research areas | >>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>Neuroactive ligand-receptor interaction, >>Adrenergic signaling in cardiomyocytes, >>Regulation of lipolysis in adipocytes, >>Renin secretion, >>Salivary secretion, >>Chemical carcinogenesis - receptor activation |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Adrenergic Receptor beta2 around the phosphorylation site of Ser346. AA range:321-370 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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