Primary Antibody ISO 9001|ABMIUM Verified™
ABM98479

Six5 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Six5 rabbit pAb from ELK Biotechnology Read more ›

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Six5 rabbit pAb
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Six5 rabbit pAb

Product Description

Six5 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Six5.

Background

The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2. [provided by RefSeq, Jul 2009],

Additional antibody information

Specificity Six5 Polyclonal Antibody detects endogenous levels of Six5 protein.
Validation evidence captions Western Blot analysis of K562 cells using Six5 Polyclonal Antibody | Western blot analysis of lysates from K562 cells, using SIX5 Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

Key Facts

Product type Primary Antibody Target: Six5
Target Six5
Also known as SIX5; DMAHP; Homeobox protein SIX5; DM locus-associated homeodomain protein; Sine oculis homeobox homolog 5
Concentration 1 mg/ml
Working concentration WB 1:500-2000;IHC-p 1:50-300
Species reactivity Human;Mouse
Observed band 75kD
Cellular localization Cytoplasm . Nucleus .
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Research area Antibody Research
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBIHC
Dilution range WB 1:500-2000;IHC-p 1:50-300
Immunogen The antiserum was produced against synthesized peptide derived from human SIX5. AA range:201-250
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000;IHC-p 1:50-300
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Six5
Also known as
SIX5; DMAHP; Homeobox protein SIX5; DM locus-associated homeodomain protein; Sine oculis homeobox homolog 5
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBIHC
Immunogen
The antiserum was produced against synthesized peptide derived from human SIX5. AA range:201-250
Observed band
75kD
Cellular localization
Cytoplasm . Nucleus .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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