Casein Kinase IIβ (phospho Ser209) rabbit pAb from ELK Biotechnology Read more ›
Casein Kinase IIβ (phospho Ser209) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Casein Kinase IIβ in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013],
| Specificity | Phospho-Casein Kinase IIβ (S209) Polyclonal Antibody detects endogenous levels of Casein Kinase IIβ protein only when phosphorylated at S209. |
|---|---|
| Validation evidence captions | Western Blot analysis of HuvEc cells using Phospho-Casein Kinase IIβ (S209) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CKII-beta (Phospho-Ser209) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using CKII-beta (Phospho-Ser209) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HUVEC cells, using CKII-beta (Phospho-Ser209) Antibody. The lane on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4865 |
| Target | Casein Kinase IIβ |
| Research areas | >>Ribosome biogenesis in eukaryotes, >>NF-kappa B signaling pathway, >>Mitophagy - animal, >>Wnt signaling pathway, >>Adherens junction, >>Alzheimer disease, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Measles, >>PD-L1 expression and PD-1 checkpoint pathway in cancer |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CKII-beta around the phosphorylation site of Ser209. AA range:166-215 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Epithelium, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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