Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98470

Casein Kinase Iδ rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Casein Kinase Iδ rabbit pAb from ELK Biotechnology Read more ›

Casein Kinase Iδ rabbit pAb
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Casein Kinase Iδ rabbit pAb

Product Description

Casein Kinase Iδ rabbit pAb is a research-use antibody from ELK Biotechnology for studying Casein Kinase Iδ in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a member of the casein kinase I (CKI) gene family whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. The encoded protein is highly similar to the mouse and rat CK1 delta homologs. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014],

Additional antibody information

Specificity Casein Kinase Iδ Polyclonal Antibody detects endogenous levels of Casein Kinase Iδ protein.
Validation evidence captions Western Blot analysis of brain cells using Casein Kinase Iδ Polyclonal Antibody | Western blot analysis of CSNK1D Antibody. The lane on the right is blocked with the CSNK1D peptide.

Key Facts

Product typePrimary Antibody Target: Casein Kinase Iδ
Collaborator ELK Biotechnology
Partner model ES4858
Target Casein Kinase Iδ
Research areas>>Hedgehog signaling pathway, >>Hippo signaling pathway, >>Gap junction, >>Circadian rhythm
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CSNK1D. AA range:291-340
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. However, kinase activity is not uniform, with highest kinase activity in splenocytes. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Casein Kinase Iδ
Also known as
CSNK1D; HCKID; Casein kinase I isoform delta; CKI-delta; CKId; Tau-protein kinase CSNK1D
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CSNK1D. AA range:291-340
Observed band
47kD
Cellular localization
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, perinuclear region. Cell membrane. Cytoplasm, cytoskeleton, spindle. Golgi apparatus. Localized at mitotic spindle microtubules, and at the centrosomes and interphase in interphase cells. Recruited to the spindle apparatus and the centrosomes in response to DNA-damage. Correct subcellular localization requires kinase activity.
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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