Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98463

GCNT7 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GCNT7 rabbit pAb from ELK Biotechnology Read more ›

GCNT7 rabbit pAb
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GCNT7 rabbit pAb

Product Description

GCNT7 rabbit pAb is a research-use antibody from ELK Biotechnology for studying GCNT7 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

function:Glycosyltransferase.,pathway:Protein modification; protein glycosylation.,sequence caution:Translated as Gln.,similarity:Belongs to the glycosyltransferase 14 family.,

Additional antibody information

Specificity GCNT7 Polyclonal Antibody detects endogenous levels of GCNT7 protein.
Validation evidence captions Immunofluorescence analysis of MCF7 cells, using GCNT7 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human ovary tissue, using GCNT7 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from K562 cells, using GCNT7 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using GCNT7 antibody.

Key Facts

Product typePrimary Antibody Target: GCNT7
Collaborator ELK Biotechnology
Partner model ES4825
Target GCNT7
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GCNT7. AA range:311-360
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Testis,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
GCNT7
Also known as
GCNT7; C20orf105; Beta-1; 3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 7
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GCNT7. AA range:311-360
Observed band
49kD
Cellular localization
Golgi apparatus membrane ; Single-pass type II membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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