Claudin-7 (phospho Tyr210) rabbit pAb
Claudin-7 (phospho Tyr210) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Claudin-7 (phospho Tyr210) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Claudin 7.
Background
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. Differential expression of this gene has been observed in different types of malignancies, including breast cancer, ovarian cancer, hepatocellular carcinomas, urinary tumors, prostate cancer, lung cancer, head and neck cancers, thyroid carcinomas, etc.. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010],
Additional antibody information
| Specificity | Phospho-Claudin-7 (Y210) Polyclonal Antibody detects endogenous levels of Claudin-7 protein only when phosphorylated at Y210. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from rat liver, using Claudin 7 (Phospho-Tyr210) Antibody. The lane on the right is blocked with the phospho peptide. |
Key Facts
| Target | Claudin 7 |
| Also known as | CLDN7; CEPTRL2; CPETRL2; Claudin-7; CLDN-7 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 32kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein . Basolateral cell membrane . Cell junction, tight junction . Co-localizes with EPCAM at the basolateral cell membrane and tight junction. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cell adhesion molecules;>>Tight junction;>>Leukocyte transendothelial migration;>>Pathogenic Escherichia coli infection;>>Hepatitis C |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Claudin 7 around the phosphorylation site of Tyr210. AA range:162-211 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.