Adenosine A2A-R rabbit pAb
Adenosine A2A-R rabbit pAb from ELK Biotechnology Read more ›
Product Description
Adenosine A2A-R rabbit pAb is an ELK Biotechnology antibody for research workflows involving Adenosine A2A-R.
Background
adenosine A2a receptor(ADORA2A) Homo sapiens This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript compos
Additional antibody information
| Specificity | Adenosine A2A-R Polyclonal Antibody detects endogenous levels of Adenosine A2A-R protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of COS7 cells, using ADORA2A Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ADORA2A Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of ADORA2A Antibody. The lane on the right is blocked with the ADORA2A peptide. | Immunohistochemistryt analysis of paraffin-embedded human brain, using ADORA2A Antibody. The lane on the right is blocked with the ADORA2A peptide. |
Key Facts
| Target | Adenosine A2A-R |
| Also known as | ADORA2A; ADORA2; Adenosine receptor A2a |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 37kD |
| Cellular localization | Cell membrane ; Multi-pass membrane protein . Colocalizes with GAS2L2 at neuronal processes. . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Rap1 signaling pathway;>>Calcium signaling pathway;>>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction;>>Vascular smooth muscle contraction;>>Parkinson disease;>>Alcoholism |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADORA2A. AA range:120-169 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.