Adenosine A2A-R rabbit pAb from ELK Biotechnology Read more ›
Adenosine A2A-R rabbit pAb is a research-use antibody from ELK Biotechnology for studying Adenosine A2A-R in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
adenosine A2a receptor(ADORA2A) Homo sapiens This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor (GPCR) superfamily, which is subdivided into classes and subtypes. The receptors are seven-pass transmembrane proteins that respond to extracellular cues and activate intracellular signal transduction pathways. This protein, an adenosine receptor of A2A subtype, uses adenosine as the preferred endogenous agonist and preferentially interacts with the G(s) and G(olf) family of G proteins to increase intracellular cAMP levels. It plays an important role in many biological functions, such as cardiac rhythm and circulation, cerebral and renal blood flow, immune function, pain regulation, and sleep. It has been implicated in pathophysiological conditions such as inflammatory diseases and neurodegenerative disorders. Alternative splicing results in multiple transcript variants. A read-through transcript compos
| Specificity | Adenosine A2A-R Polyclonal Antibody detects endogenous levels of Adenosine A2A-R protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of COS7 cells, using ADORA2A Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ADORA2A Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of ADORA2A Antibody. The lane on the right is blocked with the ADORA2A peptide. | Immunohistochemistryt analysis of paraffin-embedded human brain, using ADORA2A Antibody. The lane on the right is blocked with the ADORA2A peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4777 |
| Target | Adenosine A2A-R |
| Research areas | >>Rap1 signaling pathway, >>Calcium signaling pathway, >>cAMP signaling pathway, >>Neuroactive ligand-receptor interaction, >>Vascular smooth muscle contraction, >>Parkinson disease, >>Alcoholism |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADORA2A. AA range:120-169 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Hippocampus,Lymph,Thymus, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
