ACOT12 rabbit pAb from ELK Biotechnology Read more ›
ACOT12 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ACOT12 in IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer.,
| Specificity | ACOT12 Polyclonal Antibody detects endogenous levels of ACOT12 protein. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunofluorescence analysis of A549 cells, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4775 |
| Target | ACOT12 |
| Research areas | >>Pyruvate metabolism, >>Metabolic pathways |
| Reactivity | Human,Mouse,Rat |
| Applications | IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACOT12. AA range:281-330 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Chondrosarcoma Lung Metastasis,Liver, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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