COL4A5 rabbit pAb from ELK Biotechnology Read more ›
COL4A5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying COL4A5 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010],
| Specificity | COL4A5 Polyclonal Antibody detects endogenous levels of COL4A5 protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of HeLa cells, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from COLO205 cells using 5-HT-6 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4740 |
| Target | COL4A5 |
| Research areas | >>PI3K-Akt signaling pathway, >>Focal adhesion, >>ECM-receptor interaction, >>Relaxin signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Protein digestion and absorption, >>Amoebiasis, >>Human papillomavirus infection, >>Pathways in cancer, >>Small cell lung cancer |
| Reactivity | Human,Mouse |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen IV alpha5. AA range:21-70 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Isoform 2 is found in kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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