Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98411

Chemokine Receptor D6 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Chemokine Receptor D6 rabbit pAb from ELK Biotechnology Read more ›

Chemokine Receptor D6 rabbit pAb
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Chemokine Receptor D6 rabbit pAb

Product Description

Chemokine Receptor D6 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Chemokine Receptor D6 in IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a beta chemokine receptor, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptor-mediated signal transduction are critical for the recruitment of effector immune cells to the inflammation site. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Chemokine Receptor D6 Polyclonal Antibody detects endogenous levels of Chemokine Receptor D6 protein.
Validation evidence captions Immunofluorescence analysis of A549 cells, using CCBP2 Antibody. The picture on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: Chemokine Receptor D6
Collaborator ELK Biotechnology
Partner model ES4713
Target Chemokine Receptor D6
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications IF,ELISA
ConjugationUnconjugated
Dilution rangeImmunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CCBP2. AA range:335-384
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Found in endothelial cells lining afferent lymphatics in dermis and lymph nodes. Also found in lymph nodes subcapsular and medullary sinuses, tonsillar lymphatic sinuses and lymphatics in mucosa and submucosa of small and large intestine and appendix. Also found in some malignant vascular tumors. Expressed at high levels in Kaposi sarcoma-related pathologies. Expressed on apoptotic neutrophils (at protein level). Expressed primarily in placenta and fetal liver, and found at very low levels in the lung and lymph node.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IF
Species Dilution Notes
Human Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Rat
Mouse
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Chemokine Receptor D6
Also known as
CCBP2; CCR10; CMKBR9; Chemokine-binding protein 2; C-C chemokine receptor D6; Chemokine receptor CCR-10; Chemokine receptor CCR-9; Chemokine-binding protein D6
Concentration
1 mg/ml
Working concentration
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CCBP2. AA range:335-384
Cellular localization
Early endosome. Recycling endosome. Cell membrane; Multi-pass membrane protein. Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via clathrin-coated pits. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane.
Dilution range
Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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