Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98406

NCKX4 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

NCKX4 rabbit pAb from ELK Biotechnology Read more ›

NCKX4 rabbit pAb
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NCKX4 rabbit pAb

Product Description

NCKX4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying NCKX4 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the potassium-dependent sodium/calcium exchanger protein family. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010],

Additional antibody information

Specificity NCKX4 Polyclonal Antibody detects endogenous levels of NCKX4 protein.
Validation evidence captions Western Blot analysis of RAW264.7 cells using NCKX4 Polyclonal Antibody | Western blot analysis of lysates from RAW264.7 cells, using SLC24A4 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HeLa cells using SLC24A4 antibody.

Key Facts

Product typePrimary Antibody Target: NCKX4
Collaborator ELK Biotechnology
Partner model ES4706
Target NCKX4
Research areas>>Olfactory transduction
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human SLC24A4. AA range:328-377
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Expressed abundantly in all regions of the brain, aorta, lung and thymus (PubMed:12379639). Expressed at lower levels in the stomach and intestine (PubMed:12379639).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
NCKX4
Also known as
SLC24A4; NCKX4; Sodium/potassium/calcium exchanger 4; Na(+)/K(+)/Ca(2+)-exchange protein 4; Solute carrier family 24 member 4
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human SLC24A4. AA range:328-377
Observed band
60kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Cytoplasm .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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