PC-PLD4 rabbit pAb from ELK Biotechnology Read more ›
PC-PLD4 rabbit pAb is a research-use antibody from ELK Biotechnology for studying PLD4 in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,similarity:Belongs to the phospholipase D family.,similarity:Contains 2 PLD phosphodiesterase domains.,
| Specificity | PC-PLD4 Polyclonal Antibody detects endogenous levels of PC-PLD4 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of Jurkat cells using PC-PLD4 Polyclonal Antibody | Western blot analysis of lysates from Jurkat cells, using PLD4 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from K562 cells using PLD4 antibody. | Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
| Collaborator | ELK Biotechnology |
| Partner model | ES4701 |
| Target | PLD4 |
| Research areas | >>Glycerophospholipid metabolism, >>Ether lipid metabolism, >>Metabolic pathways |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLD4. AA range:457-506 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | B-cell,T-cell, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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