ABM98400
Casein Kinase I α1/1L rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Casein Kinase I α1/1L rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Casein Kinase I α1/1L rabbit pAb is an ELK Biotechnology antibody for research workflows involving Casein Kinase I α1/1L.
Background
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling.,similarity:Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.,similarity:Contains 1 protein kinase domain.,
Additional antibody information
| Specificity | Casein Kinase I α1/1L Polyclonal Antibody detects endogenous levels of Casein Kinase I α1/1L protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549 cells, using CKI-alpha1/L Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CKI-alpha1/L Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: Casein Kinase I α1/1L
| Target | Casein Kinase I α1/1L |
| Also known as | CSNK1A1L; Casein kinase I isoform alpha-like; CKI-alpha-like; CK1; CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 30kD |
| Cellular localization | Cytoplasm . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Wnt signaling pathway;>>Hedgehog signaling pathway;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CKI-alpha1/L. AA range:141-190 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Casein Kinase I α1/1L
Also known as
CSNK1A1L; Casein kinase I isoform alpha-like; CKI-alpha-like; CK1; CSNK1A1; Casein kinase I isoform alpha; CKI-alpha; CK1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CKI-alpha1/L. AA range:141-190
Observed band
30kD
Cellular localization
Cytoplasm .
Dilution range
WB 1:500-2000 Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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