Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM98391

CLN5 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CLN5 rabbit pAb from ELK Biotechnology Read more ›

CLN5 rabbit pAb
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CLN5 rabbit pAb

Product Description

CLN5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CLN5 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

ceroid-lipofuscinosis, neuronal 5(CLN5) Homo sapiens This gene is one of eight which have been associated with neuronal ceroid lipofuscinoses (NCL). Also referred to as Batten disease, NCL comprises a class of autosomal recessive, neurodegenerative disorders affecting children. The genes responsible likely encode proteins involved in the degradation of post-translationally modified proteins in lysosomes. The primary defect in NCL disorders is thought to be associated with lysosomal storage function.[provided by RefSeq, Oct 2008],

Additional antibody information

Specificity CLN5 Polyclonal Antibody detects endogenous levels of CLN5 protein.
Validation evidence captions Western Blot analysis of 293T cells using CLN5 Polyclonal Antibody diluted at 1:1000 | Western blot analysis of CLN5 Antibody. The lane on the right is blocked with the CLN5 peptide. | Western blot analysis of the lysates from COLO205 cells using CLN5 antibody.

Key Facts

Product typePrimary Antibody Target: CLN5
Collaborator ELK Biotechnology
Partner model ES4682
Target CLN5
Research areas>>Lysosome
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CLN5. AA range:171-220
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Ubiquitous.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CLN5
Also known as
CLN5; Ceroid-lipofuscinosis neuronal protein 5; Protein CLN5
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CLN5. AA range:171-220
Observed band
48kD
Cellular localization
Ceroid-lipofuscinosis neuronal protein 5, secreted form: Lysosome .; Ceroid-lipofuscinosis neuronal protein 5: Membrane ; Single-pass type II membrane protein . An amphipathic anchor region facilitates its association with the membrane. .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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