CYP2R1 rabbit pAb from ELK Biotechnology Read more ›
CYP2R1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CYP2R1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. [provided by RefSeq, Jul 2008],
| Specificity | CYP2R1 Polyclonal Antibody detects endogenous levels of CYP2R1 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of HT-29 cells using CYP2R1 Polyclonal Antibody | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Cytochrome P450 2R1 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HT29 cells, using Cytochrome P450 2R1 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from HT-29 cells using Cytochrome P450 2R1 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4681 |
| Target | CYP2R1 |
| Research areas | >>Steroid biosynthesis, >>Metabolic pathways |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CYP2R1. AA range:251-300 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Liver, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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