ABM98376
Rhophilin rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Rhophilin rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Rhophilin rabbit pAb is an ELK Biotechnology antibody for research workflows involving Rhophilin.
Background
domain:The PDZ domain mediates interaction with ROPN1.,function:Has no enzymatic activity. May serve as a target for Rho, and interact with some cytoskeletal component upon Rho binding or relay a Rho signal to other molecules.,similarity:Belongs to the RHPN family.,similarity:Contains 1 BRO1 domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 REM (Hr1) repeat.,subunit:Binds specifically to GTP-Rho. Interacts with ROPN1.,
Additional antibody information
| Specificity | Rhophilin Polyclonal Antibody detects endogenous levels of Rhophilin protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of A549 cells using Rhophilin Polyclonal Antibody | Western blot analysis of the lysates from HepG2 cells using RHPN1 antibody. | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: Rhophilin
| Target | Rhophilin |
| Also known as | RHPN1; KIAA1929; Rhophilin-1; GTP-Rho-binding protein 1 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 100kD |
| Cellular localization | cytosol, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCELISA |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RHPN1. AA range:474-523 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Rhophilin
Also known as
RHPN1; KIAA1929; Rhophilin-1; GTP-Rho-binding protein 1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Rat;Mouse;
Tested applications
WBIHCELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human RHPN1. AA range:474-523
Observed band
100kD
Cellular localization
cytosol,
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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