mAChR M5 rabbit pAb from ELK Biotechnology Read more ›
mAChR M5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying mAChR M5 in WB, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels. [provided by RefSeq, Jul 2008],
| Specificity | mAChR M5 Polyclonal Antibody detects endogenous levels of mAChR M5 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of Jurkat cells using mAChR M5 Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using CHRM5 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from LOVO and RAW264.7 cells, using CHRM5 Antibody. The lane on the right is blocked with the synthesized peptide. | Western blot analysis of the lysates from K562 cells using CHRM5 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4636 |
| Target | mAChR M5 |
| Research areas | >>Calcium signaling pathway, >>Neuroactive ligand-receptor interaction, >>Cholinergic synapse, >>Regulation of actin cytoskeleton, >>Alzheimer disease, >>Pathways of neurodegeneration - multiple diseases |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CHRM5. AA range:281-330 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain,Keratinocyte,Lens epithelium,Placenta,Testis, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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