MASP-2 rabbit pAb from ELK Biotechnology Read more ›
MASP-2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying MASP-2 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
mannan binding lectin serine peptidase 2(MASP2) Homo sapiens This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein is proteolytically processed to generate A and B chains that heterodimerize to form the mature protease. This protease cleaves complement components C2 and C4 in order to generate C3 convertase in the lectin pathway of the complement system. The encoded protease also plays a role in the coagulation cascade through cleavage of prothrombin to form thrombin. Myocardial infarction and acute stroke patients exhibit reduced serum concentrations of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016],
| Specificity | MASP-2 Polyclonal Antibody detects endogenous levels of MASP-2 protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of K562 cells using MASP-2 Polyclonal Antibody | Western blot analysis of MASP2 Antibody. The lane on the right is blocked with the MASP2 peptide. | Western blot analysis of the lysates from HT-29 cells using MASP2 antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4548 |
| Target | MASP-2 |
| Research areas | >>Complement and coagulation cascades, >>Staphylococcus aureus infection, >>Coronavirus disease - COVID-19 |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MASP2. AA range:227-276 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Plasma. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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