ABM98326
Six2 rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Six2 rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Six2 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Six2.
Background
This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development. [provided by RefSeq, Dec 2008],
Additional antibody information
| Specificity | Six2 Polyclonal Antibody detects endogenous levels of Six2 protein. |
|---|---|
| Validation evidence captions | Western blot analysis of SIX2 Antibody. The lane on the right is blocked with the SIX2 peptide. | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). |
Key Facts
Product type
Primary Antibody›
Target: Six2
| Target | Six2 |
| Also known as | SIX2; Homeobox protein SIX2; Sine oculis homeobox homolog 2 |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000;IHC-p 1:50-300 |
| Species reactivity | Human;Mouse |
| Cellular localization | Nucleus . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Immunogen | Synthesized peptide derived from Six2 . at AA range: 30-110 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
2 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000;IHC-p 1:50-300 | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Six2
Also known as
SIX2; Homeobox protein SIX2; Sine oculis homeobox homolog 2
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse
Tested applications
WBIHC
Immunogen
Synthesized peptide derived from Six2 . at AA range: 30-110
Cellular localization
Nucleus .
Dilution range
WB 1:500-2000;IHC-p 1:50-300
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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