ABM98322
DNAM-1 (phospho Ser329) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
DNAM-1 (phospho Ser329) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
DNAM-1 (phospho Ser329) rabbit pAb is an ELK Biotechnology antibody for research workflows involving DNAM-1.
Background
This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],
Additional antibody information
| Specificity | Phospho-DNAM-1 (S329) Polyclonal Antibody detects endogenous levels of DNAM-1 protein only when phosphorylated at S329. |
|---|---|
| Validation evidence captions | Western Blot analysis of COS7 cells using Phospho-DNAM-1 (S329) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD226/DNAM-1 (Phospho-Ser329) Antibody | Immunofluorescence analysis of A549 cells, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide. |
Key Facts
Product type
Primary Antibody›
Target: DNAM-1
| Target | DNAM-1 |
| Also known as | CD226; DNAM1; CD226 antigen; DNAX accessory molecule 1; DNAM-1; CD antigen CD226 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Monkey |
| Observed band | 60kD |
| Cellular localization | Cell membrane ; Single-pass type I membrane protein . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Cell adhesion molecules |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD226/DNAM-1 around the phosphorylation site of Ser329. AA range:287-336 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
DNAM-1
Also known as
CD226; DNAM1; CD226 antigen; DNAX accessory molecule 1; DNAM-1; CD antigen CD226
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Monkey
Tested applications
WBIHCIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CD226/DNAM-1 around the phosphorylation site of Ser329. AA range:287-336
Observed band
60kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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