DNAM-1 (phospho Ser329) rabbit pAb from ELK Biotechnology Read more ›
DNAM-1 (phospho Ser329) rabbit pAb is a research-use antibody from ELK Biotechnology for studying DNAM-1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],
| Specificity | Phospho-DNAM-1 (S329) Polyclonal Antibody detects endogenous levels of DNAM-1 protein only when phosphorylated at S329. |
|---|---|
| Validation evidence captions | Western Blot analysis of COS7 cells using Phospho-DNAM-1 (S329) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD226/DNAM-1 (Phospho-Ser329) Antibody | Immunofluorescence analysis of A549 cells, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using CD226/DNAM-1 (Phospho-Ser329) Antibody. The picture on the right is blocked with the phospho peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4531 |
| Target | DNAM-1 |
| Research areas | >>Cell adhesion molecules |
| Reactivity | Human,Mouse,Monkey |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD226/DNAM-1 around the phosphorylation site of Ser329. AA range:287-336 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed by peripheral blood T-lymphocytes. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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